› 2hlp

X-ray diffraction
2.59Å resolution

CRYSTAL STRUCTURE OF THE E267R MUTANT OF A HALOPHILIC MALATE DEHYDROGENASE IN THE APO FORM

Released:
DOI: 10.2210/pdb2hlp/pdb
PDB entry 2hlp coloured by chain, front view.
PDB entry 2hlp coloured by chain, side view.
PDB entry 2hlp coloured by chain, top view.
Source organism: Haloarcula marismortui
Primary publication:
Halophilic adaptation: novel solvent protein interactions observed in the 2.9 and 2.6 A resolution structures of the wild type and a mutant of malate dehydrogenase from Haloarcula marismortui.
Richard SB, Madern D, Garcin E, Zaccai G
Biochemistry 39 992-1000 (2000)
PMID: 10653643

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 32.73 KDa
Source organism: Haloarcula marismortui
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07841 (Residues: 2-304; Coverage: 100%)
Gene names: mdh, rrnAC2706
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: C2221
Unit cell:
a: 114.16Å b: 129.98Å c: 123.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.236
Expression system: Escherichia coli

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Citations

4 review citations

Halophilic enzymes: proteins with a grain of salt.
Mevarech et al. (2000)
3 more