› 2hmp

X-ray diffraction
1.9Å resolution

Uncomplexed actin cleaved with protease ECP32

Released:
DOI: 10.2210/pdb2hmp/pdb
PDB entry 2hmp coloured by chain, front view.
PDB entry 2hmp coloured by chain, side view.
PDB entry 2hmp coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Crystal structure of polymerization-competent actin.
Klenchin VA, Khaitlina SY, Rayment I
J Mol Biol 362 140-50 (2006)
PMID: 16893553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SR 7 x SR
Ligand SPD 1 x SPD
Ligand ATP 2 x ATP
Ligand EDO 9 x EDO
Ligand 211 2 x 211
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 63.9Å b: 198.11Å c: 69.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.215

Quick links

Citations

4 review citations

The nucleoskeleton as a genome-associated dynamic 'network of networks'.
Simon et al. (2011)
3 more

3 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
2 more