› 2hmy

X-ray diffraction
2.61Å resolution

BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE

Released:
DOI: 10.2210/pdb2hmy/pdb
PDB entry 2hmy coloured by chain, front view.
PDB entry 2hmy coloured by chain, side view.
PDB entry 2hmy coloured by chain, top view.
Source organism: Haemophilus haemolyticus
Primary publication:
Structure of a binary complex of HhaI methyltransferase with S-adenosyl-L-methionine formed in the presence of a short non-specific DNA oligonucleotide.
O'Gara M, Zhang X, Roberts RJ, Cheng X
J Mol Biol 287 201-9 (1999)
PMID: 10080885

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M.HhaI Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 37.04 KDa
Source organism: Haemophilus haemolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05102 (Residues: 1-327; Coverage: 100%)
Gene name: hhaIM
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 55.3Å b: 74.68Å c: 90.96Å
α: 90° β: 103° γ: 90°
R-values:
R R work R free
0.198 0.198 not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

5-Azacytidine and 5-aza-2'-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy.
Christman JK. (2002)
3 more

16 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
15 more