› 2ho7

X-ray diffraction
2.9Å resolution

Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme bound to glucose-6-phosphate

Released:
DOI: 10.2210/pdb2ho7/pdb
PDB entry 2ho7 coloured by chain, front view.
PDB entry 2ho7 coloured by chain, side view.
PDB entry 2ho7 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate.
Klein DJ, Ferré-D'Amaré AR
Science 313 1752-6 (2006)
PMID: 16990543

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195870 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
glmS ribozyme substrate RNA Chain: A
Molecule details ›
Chain: A
Length: 27 nucleotides
Theoretical weight: 8.61 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
glmS ribozyme RNA Chain: B
Molecule details ›
Chain: B
Length: 125 nucleotides
Theoretical weight: 40.6 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Not provided
Sequence domains: glmS glucosamine-6-phosphate activated ribozyme

Ligands and Environments

2 bound ligands:
Ligand G6P 1 x G6P
Ligand MG 4 x MG
1 modified residue:
Ligand A23 1 x A23

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 181.443Å b: 40.84Å c: 72.097Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.239
Expression system: Not provided

Quick links

Citations

93 review citations

A decade of riboswitches.
Serganov et al. (2013)
92 more

9 mentions without citation

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
8 more