› 2hop

X-ray diffraction
3.3Å resolution

Crystal structure of an E. coli thi-box riboswitch bound to pyrithiamine

Released:
DOI: 10.2210/pdb2hop/pdb
PDB entry 2hop coloured by chain, front view.
PDB entry 2hop coloured by chain, side view.
PDB entry 2hop coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition.
Edwards TE, Ferré-D'Amaré AR
Structure 14 1459-68 (2006)
PMID: 16962976

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195845 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
thi-box riboswitch Chain: A
Molecule details ›
Chain: A
Length: 83 nucleotides
Theoretical weight: 26.8 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: TPP riboswitch (THI element)

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand CA 1 x CA
Ligand 218 1 x 218
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3212
Unit cell:
a: 61.3Å b: 61.3Å c: 103.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.251 0.331
Expression system: Not provided

Quick links

Citations

47 review citations

A decade of riboswitches.
Serganov et al. (2013)
46 more

10 mentions without citation

Structure and function of the T-loop structural motif in noncoding RNAs.
Chan et al. (2013)
9 more