› 2hq5

X-ray diffraction
2.8Å resolution

Crystal structure of multidrug binding protein QacR from Staphylococcus aureus cocrystallized with compound DB359

Released:
DOI: 10.2210/pdb2hq5/pdb
PDB entry 2hq5 coloured by chain, front view.
PDB entry 2hq5 coloured by chain, side view.
PDB entry 2hq5 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Multidrug-binding transcription factor QacR binds the bivalent aromatic diamidines DB75 and DB359 in multiple positions.
Brooks BE, Piro KM, Brennan RG
J Am Chem Soc 129 8389-95 (2007)
PMID: 17567017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 194 amino acids
Theoretical weight: 22.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P42212
Unit cell:
a: 171.366Å b: 171.366Å c: 94.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.259
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Multidrug resistance in bacteria.
Nikaido H. (2009)
3 more

1 mention without citation

The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences.
Newman et al. (2021)