› 2hqa

X-ray diffraction
2.6Å resolution

Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III

Released:
DOI: 10.2210/pdb2hqa/pdb
PDB entry 2hqa coloured by chain, front view.
PDB entry 2hqa coloured by chain, side view.
PDB entry 2hqa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the catalytic alpha subunit of E. coli replicative DNA polymerase III.
Lamers MH, Georgescu RE, Lee SG, O'Donnell M, Kuriyan J
Cell 126 881-92 (2006)
PMID: 16959568

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 917 amino acids
Theoretical weight: 103.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P10443 (Residues: 1-917; Coverage: 79%)
Gene names: JW0179, b0184, dnaE, polC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 83.112Å b: 98.264Å c: 139.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.287
Expression system: Escherichia coli

Quick links

Citations

20 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
19 more

7 mentions without citation

Replisome dynamics and use of DNA trombone loops to bypass replication blocks.
Yao et al. (2008)
6 more