› 2hqk

X-ray diffraction
1.19Å resolution

Crystal structure of a monomeric cyan fluorescent protein derived from Clavularia

Released:
DOI: 10.2210/pdb2hqk/pdb
PDB entry 2hqk coloured by chain, front view.
PDB entry 2hqk coloured by chain, side view.
PDB entry 2hqk coloured by chain, top view.
Source organism: Clavularia sp.
Primary publication:
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
Ai HW, Henderson JN, Remington SJ, Campbell RE
Biochem J 400 531-40 (2006)
PMID: 16859491

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein cFP484 Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.18 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y3 (Residues: 44-264; Coverage: 83%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand ZN 3 x ZN
Ligand CL 1 x CL
Ligand ACT 1 x ACT
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 89.83Å b: 38.02Å c: 61.119Å
α: 90° β: 90.81° γ: 90°
R-values:
R R work R free
0.149 0.137 0.206
Expression system: Escherichia coli

Quick links

Citations

57 review citations

Fluorescence lifetime measurements and biological imaging.
Berezin et al. (2010)
56 more

23 mentions without citation

Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.
Ai et al. (2006)
22 more