› 2hqp

Solution NMR

Solution structure of L.casei dihydrofolate reductase complexed with NADPH, 32 structures

Released:
DOI: 10.2210/pdb2hqp/pdb
PDB entry 2hqp coloured by chain, ensemble of 32 models, front view.
PDB entry 2hqp coloured by chain, ensemble of 32 models, side view.
PDB entry 2hqp coloured by chain, ensemble of 32 models, top view.
Source organism: Lacticaseibacillus casei
Entry authors: Polshakov VI, Birdsall B

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.33 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: P00381 (Residues: 2-163; Coverage: 99%)
Gene names: dhfR, folA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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