› 2hra

X-ray diffraction
1.9Å resolution

Crystal structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA aminoacylation and nuclear export cofactor Arc1p reveal a novel function for an old fold

Released:
DOI: 10.2210/pdb2hra/pdb
PDB entry 2hra coloured by chain, front view.
PDB entry 2hra coloured by chain, side view.
PDB entry 2hra coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold.
Simader H, Hothorn M, Suck D
Acta Crystallogr D Biol Crystallogr 62 1510-9 (2006)
PMID: 17139087

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 22.96 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46655 (Residues: 1-191; Coverage: 27%)
Gene names: G0583, GUS1, HRB724, YGL245W
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IOD 44 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1, SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 51.959Å b: 107.113Å c: 167.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.262
Expression system: Escherichia coli

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Citations

2 review citations

Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.
Hausmann et al. (2008)
1 more

2 mentions without citation

Protein-protein docking benchmark version 3.0.
Hwang et al. (2008)
1 more