2ht2

X-ray diffraction
3.32Å resolution

Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211167 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Ig-like domain-containing protein Chains: C, E
Molecule details ›
Chains: C, E
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q4VBH1 (Residues: 21-126, 127-241; Coverage: 49%)
Gene name: Ighg
Sequence domains:
Structure domains: Immunoglobulins
Fab fragment, light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 232.294Å b: 96.58Å c: 170.557Å
α: 90° β: 131.43° γ: 90°
R-values:
R R work R free
0.272 0.27 0.305
Expression system: Escherichia coli BL21(DE3)