› 2ht6

X-ray diffraction
2.4Å resolution

Crystal structure of Human Gem G-domain bound to GDP

Released:
DOI: 10.2210/pdb2ht6/pdb
PDB entry 2ht6 coloured by chain, front view.
PDB entry 2ht6 coloured by chain, side view.
PDB entry 2ht6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function studies of the G-domain from human gem, a novel small G-protein.
Opatowsky Y, Sasson Y, Shaked I, Ward Y, Chomsky-Hecht O, Litvak Y, Selinger Z, Kelly K, Hirsch JA
FEBS Lett 580 5959-64 (2006)
PMID: 17052716

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein GEM Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55040 (Residues: 71-243; Coverage: 58%)
Gene names: GEM, KIR
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 39.843Å b: 81.433Å c: 124.095Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.287
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
7 more

4 mentions without citation

RGK regulation of voltage-gated calcium channels.
Buraei et al. (2015)
3 more