› 2htn

X-ray diffraction
2.5Å resolution

E. coli bacterioferritin in its as-isolated form

Released:
DOI: 10.2210/pdb2htn/pdb
PDB entry 2htn coloured by chain, front view.
PDB entry 2htn coloured by chain, side view.
PDB entry 2htn coloured by chain, top view.
Source organism: Escherichia coli BL21
Primary publication:
Fortuitous structure determination of 'as-isolated' Escherichia coli bacterioferritin in a novel crystal form.
van Eerde A, Wolterink-van Loo S, van der Oost J, Dijkstra BW
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 1061-6 (2006)
PMID: 17077480

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 158 amino acids
Theoretical weight: 18.52 KDa
Source organism: Escherichia coli BL21
UniProt:
  • Canonical: P0ABD3 (Residues: 1-158; Coverage: 100%)
Gene names: JW3298, b3336, bfr
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

2 bound ligands:
Ligand FE 16 x FE
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P213
Unit cell:
a: 167.893Å b: 167.893Å c: 167.893Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.188

Quick links

Citations

1 citations in other articles

The binding of haem and zinc in the 1.9 A X-ray structure of Escherichia coli bacterioferritin.
Willies et al. (2009)

3 mentions without citation

Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem.
Gupta et al. (2009)
2 more