› 2hun

X-ray diffraction
2.07Å resolution

Crystal structure of hypothetical protein PH0414 from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2hun/pdb
PDB entry 2hun coloured by chain, front view.
PDB entry 2hun coloured by chain, side view.
PDB entry 2hun coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Yamamoto H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 38.98 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58151 (Residues: 1-336; Coverage: 100%)
Gene name: PH0414
Sequence domains: GDP-mannose 4,6 dehydratase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 142.892Å b: 73.677Å c: 100.222Å
α: 90° β: 128.995° γ: 90°
R-values:
R R work R free
0.204 0.204 0.232
Expression system: Escherichia coli

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