› 2hve

X-ray diffraction
2.4Å resolution

S120G mutant of human nucleoside diphosphate kinase A complexed with ADP

Released:
DOI: 10.2210/pdb2hve/pdb
PDB entry 2hve coloured by chain, front view.
PDB entry 2hve coloured by chain, side view.
PDB entry 2hve coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of S120G mutant and wild type of human nucleoside diphosphate kinase A in complex with ADP.
Giraud MF, Georgescauld F, Lascu I, Dautant A
J Bioenerg Biomembr 38 261-4 (2006)
PMID: 16944299

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147400 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 152 amino acids
Theoretical weight: 17.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15531 (Residues: 1-152; Coverage: 100%)
Gene names: NDPKA, NM23, NME1
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P41212
Unit cell:
a: 114.824Å b: 114.824Å c: 89.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.277
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The mammalian Nm23/NDPK family: from metastasis control to cilia movement.
Boissan et al. (2009)
7 more

6 mentions without citation

Arylfluorosulfate-Based Electrophiles for Covalent Protein Labeling: A New Addition to the Arsenal.
Martín-Gago et al. (2019)
5 more