› 2hvj

X-ray diffraction
2.75Å resolution

Crystal structure of KcsA-Fab-TBA complex in low K+

Released:
DOI: 10.2210/pdb2hvj/pdb
PDB entry 2hvj coloured by chain, front view.
PDB entry 2hvj coloured by chain, side view.
PDB entry 2hvj coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel.
Yohannan S, Hu Y, Zhou Y
J Mol Biol 366 806-14 (2007)
PMID: 17196615

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209454 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody Fab heavy chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
antibody Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.21 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-124; Coverage: 78%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

4 bound ligands:
Ligand F09 1 x F09
Ligand K 2 x K
Ligand L2C 1 x L2C
Ligand TBA 1 x TBA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4
Unit cell:
a: 156.423Å b: 156.423Å c: 75.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.246
Expression system: Escherichia coli

Quick links

Citations

8 review citations

K+ channel modulators for the treatment of neurological disorders and autoimmune diseases.
Wulff et al. (2008)
7 more

3 mentions without citation

Accessibility of Cations to the Selectivity Filter of KcsA in the Inactivated State: An Equilibrium Binding Study.
Giudici et al. (2019)
2 more