› 2hw4

X-ray diffraction
1.9Å resolution

Crystal structure of human phosphohistidine phosphatase

Released:
DOI: 10.2210/pdb2hw4/pdb
PDB entry 2hw4 coloured by chain, front view.
PDB entry 2hw4 coloured by chain, side view.
PDB entry 2hw4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
First structure of a eukaryotic phosphohistidine phosphatase.
Busam RD, Thorsell AG, Flores A, Hammarström M, Persson C, Hallberg BM
J Biol Chem 281 33830-4 (2006)
PMID: 16990267

Function and Biology Details

Reaction catalysed: 
A [protein]-N-phospho-L-histidine + H(2)O = a [protein]-L-histidine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14 kDa phosphohistidine phosphatase Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRX4 (Residues: 5-125; Coverage: 97%)
Gene names: CGI-202, HSPC141, PHP14, PHPT1
Sequence domains: Janus/Ocnus family (Ocnus)
Structure domains: Janus/Ocnus

Ligands and Environments

1 bound ligand:
Ligand FMT 17 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R3
Unit cell:
a: 112.53Å b: 112.53Å c: 29.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.171 0.225
Expression system: Escherichia coli

Quick links

Citations

6 review citations

pHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification.
Fuhs et al. (2017)
5 more

2 mentions without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)
1 more