› 2hwg

X-ray diffraction
2.7Å resolution

Structure of phosphorylated Enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system

Released:
DOI: 10.2210/pdb2hwg/pdb
PDB entry 2hwg coloured by chain, front view.
PDB entry 2hwg coloured by chain, side view.
PDB entry 2hwg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of phosphorylated enzyme I, the phosphoenolpyruvate:sugar phosphotransferase system sugar translocation signal protein.
Teplyakov A, Lim K, Zhu PP, Kapadia G, Chen CC, Schwartz J, Howard A, Reddy PT, Peterkofsky A, Herzberg O
Proc Natl Acad Sci U S A 103 16218-23 (2006)
PMID: 17053069

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate-protein phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 575 amino acids
Theoretical weight: 64.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P08839 (Residues: 1-575; Coverage: 100%)
Gene names: JW2409, b2416, ptsI
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand OXL 2 x OXL
2 modified residues:
Ligand MSE 36 x MSE
Ligand NEP 2 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 85.487Å b: 94.084Å c: 161.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.284
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, dynamics and biophysics of the cytoplasmic protein-protein complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system.
Clore et al. (2013)
3 more

22 mentions without citation

Hybrid Approaches to Structural Characterization of Conformational Ensembles of Complex Macromolecular Systems Combining NMR Residual Dipolar Couplings and Solution X-ray Scattering.
Venditti et al. (2016)
21 more