› 2hyj

X-ray diffraction
2.19Å resolution

The crystal structure of a tetR-family transcriptional regulator from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb2hyj/pdb
PDB entry 2hyj coloured by chain, front view.
PDB entry 2hyj coloured by chain, side view.
PDB entry 2hyj coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Entry authors: Zhang R, Xu X, Zheng H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 21.77 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CJS4 (Residues: 1-200; Coverage: 100%)
Gene names: SCK13.32, SCO4940
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 32.864Å b: 82.678Å c: 178.933Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.247
Expression system: Escherichia coli BL21(DE3)

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