› 2hyk

X-ray diffraction
1.3Å resolution

The crystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96

Released:
DOI: 10.2210/pdb2hyk/pdb
PDB entry 2hyk coloured by chain, front view.
PDB entry 2hyk coloured by chain, side view.
PDB entry 2hyk coloured by chain, top view.
Source organism: Nocardiopsis sp. F96
Primary publication:
The 1.3 A crystal structure of a novel endo-beta-1,3-glucanase of glycoside hydrolase family 16 from alkaliphilic Nocardiopsis sp. strain F96.
Fibriansah G, Masuda S, Koizumi N, Nakamura S, Kumasaka T
Proteins 69 683-90 (2007)
PMID: 17879342

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH16 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.13 KDa
Source organism: Nocardiopsis sp. F96
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2V0H0 (Residues: 26-270; Coverage: 99%)
Gene name: bglF
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand CA 1 x CA
Ligand EOH 2 x EOH
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 34.589Å b: 71.843Å c: 39.666Å
α: 90° β: 90.21° γ: 90°
R-values:
R R work R free
0.144 0.143 0.162
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Nocardiopsis species: Incidence, ecological roles and adaptations.
Bennur et al. (2015)
2 more

13 mentions without citation

Targeting an antimicrobial effector function in insect immunity as a pest control strategy.
Bulmer et al. (2009)
12 more