› 2hyr

X-ray diffraction
1.51Å resolution

Crystal structure of a complex of griffithsin with maltose

Released:
DOI: 10.2210/pdb2hyr/pdb
PDB entry 2hyr coloured by chain, front view.
PDB entry 2hyr coloured by chain, side view.
PDB entry 2hyr coloured by chain, top view.
Source organism: Griffithsia
Primary publication:
Crystallographic, thermodynamic, and molecular modeling studies of the mode of binding of oligosaccharides to the potent antiviral protein griffithsin.
Ziółkowska NE, Shenoy SR, O'Keefe BR, McMahon JB, Palmer KE, Dwek RA, Wormald MR, Wlodawer A
Proteins 67 661-70 (2007)
PMID: 17340634

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Griffithsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 12.73 KDa
Source organism: Griffithsia
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: P84801 (Residues: 1-121; Coverage: 100%)
Sequence domains: Jacalin-like lectin domain
Structure domains: Jacalin-like lectin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
3 bound ligands:
Ligand SO4 3 x SO4
Ligand MG 2 x MG
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 34.82Å b: 53.26Å c: 118.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.185
Expression system: Nicotiana benthamiana

Quick links

Citations

12 review citations

Binding sugars: from natural lectins to synthetic receptors and engineered neolectins.
Arnaud et al. (2013)
11 more

4 mentions without citation

Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties.
Barre et al. (2019)
3 more