› 2hza

X-ray diffraction
2.1Å resolution

Nickel-bound full-length Escherichia coli NikR

Released:
DOI: 10.2210/pdb2hza/pdb
PDB entry 2hza coloured by chain, front view.
PDB entry 2hza coloured by chain, side view.
PDB entry 2hza coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
NikR-operator complex structure and the mechanism of repressor activation by metal ions.
Schreiter ER, Wang SC, Zamble DB, Drennan CL
Proc Natl Acad Sci U S A 103 13676-81 (2006)
PMID: 16945905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Z6 (Residues: 1-133; Coverage: 100%)
Gene names: JW3446, b3481, nikR, yhhG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand 3CM 1 x 3CM
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 49.843Å b: 49.843Å c: 181.691Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.274
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
17 more

11 mentions without citation

Allosteric control of metal-responsive transcriptional regulators in bacteria.
Baksh et al. (2020)
10 more