› 2hze

X-ray diffraction
1.8Å resolution

Crystal structures of a poxviral glutaredoxin in the oxidized and reduced states show redox-correlated structural changes

Released:
DOI: 10.2210/pdb2hze/pdb
PDB entry 2hze coloured by chain, front view.
PDB entry 2hze coloured by chain, side view.
PDB entry 2hze coloured by chain, top view.
Source organism: Ectromelia virus Moscow
Primary publication:
Crystal structures of a poxviral glutaredoxin in the oxidized and reduced states show redox-correlated structural changes.
Bacik JP, Hazes B
J Mol Biol 365 1545-58 (2007)
PMID: 17137595

Function and Biology Details

Reaction catalysed: 
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutaredoxin-1 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.16 KDa
Source organism: Ectromelia virus Moscow
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JLF5 (Residues: 1-108; Coverage: 100%)
Gene names: EVM053, OPG075
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin
Glutaredoxin-1 Chain: B
Molecule details ›
Chain: B
Length: 114 amino acids
Theoretical weight: 13.26 KDa
Source organism: Ectromelia virus Moscow
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JLF5 (Residues: 1-108; Coverage: 100%)
Gene names: EVM053, OPG075
Sequence domains: Glutaredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CAS 1 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 62.627Å b: 66.668Å c: 108.096Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
1 more

4 mentions without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)
3 more