2hzm

X-ray diffraction
2.4Å resolution

Structure of the Mediator head subcomplex Med18/20

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-152374 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 20 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 212 amino acids
Theoretical weight: 23.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34162 (Residues: 1-210; Coverage: 100%)
Gene names: HRS2, MED20, SRB2, YHR041C
Sequence domains: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Structure domains:
Mediator of RNA polymerase II transcription subunit 18 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 317 amino acids
Theoretical weight: 35.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32585 (Residues: 2-307; Coverage: 100%)
Gene names: MED18, SRB5, YGR104C
Sequence domains: Med18 protein
Structure domains: Hypothetical Protein Pfu-838710-001

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 72.903Å b: 129.352Å c: 241.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.264
Expression system: Escherichia coli