› 2hzs

X-ray diffraction
2.7Å resolution

Structure of the Mediator head submodule Med8C/18/20

Released:
DOI: 10.2210/pdb2hzs/pdb
PDB entry 2hzs coloured by chain, front view.
PDB entry 2hzs coloured by chain, side view.
PDB entry 2hzs coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.
Larivière L, Geiger S, Hoeppner S, Röther S, Strässer K, Cramer P
Nat Struct Mol Biol 13 895-901 (2006)
PMID: 16964259

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
hetero nonamer
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-152375 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mediator of RNA polymerase II transcription subunit 20 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 209 amino acids
Theoretical weight: 22.79 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34162 (Residues: 2-210; Coverage: 100%)
Gene names: HRS2, MED20, SRB2, YHR041C
Sequence domains: TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Structure domains:
Mediator of RNA polymerase II transcription subunit 18 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 306 amino acids
Theoretical weight: 34.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32585 (Residues: 2-307; Coverage: 100%)
Gene names: MED18, SRB5, YGR104C
Sequence domains: Med18 protein
Structure domains: Hypothetical Protein Pfu-838710-001
Mediator of RNA polymerase II transcription subunit 8 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 27 amino acids
Theoretical weight: 3.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38304 (Residues: 190-210; Coverage: 9%)
Gene names: MED8, YBR1403, YBR193C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 75.365Å b: 115.762Å c: 129.159Å
α: 90° β: 98.49° γ: 90°
R-values:
R R work R free
0.236 0.236 0.272
Expression system: Escherichia coli

Quick links

Citations

21 review citations

The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation.
Malik et al. (2010)
20 more

3 mentions without citation

Disordered proteinaceous machines.
Fuxreiter et al. (2014)
2 more