› 2i00

X-ray diffraction
2.3Å resolution

Crystal structure of acetyltransferase (GNAT family) from Enterococcus faecalis

Released:
DOI: 10.2210/pdb2i00/pdb
PDB entry 2i00 coloured by chain, front view.
PDB entry 2i00 coloured by chain, side view.
PDB entry 2i00 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Zhang R, Zhou M, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-182778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 406 amino acids
Theoretical weight: 48.08 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q831Y9 (Residues: 1-406; Coverage: 100%)
Gene name: EF_2353
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 77.41Å b: 177.091Å c: 104.429Å
α: 90° β: 94.89° γ: 90°
R-values:
R R work R free
0.216 0.213 0.27
Expression system: Escherichia coli BL21

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