› 2i0m

X-ray diffraction
2.4Å resolution

Crystal structure of the phosphate transport system regulatory protein PhoU from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb2i0m/pdb
PDB entry 2i0m coloured by chain, front view.
PDB entry 2i0m coloured by chain, side view.
PDB entry 2i0m coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Entry authors: Zhang R, Li H, Abdullah J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-specific transport system accessory protein PhoU homolog Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.21 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A3Y7 (Residues: 1-216; Coverage: 100%)
Gene names: SP_2088, phoU
Sequence domains: PhoU domain
Structure domains: Phosphate transport system protein phou homolog 2; domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 72.547Å b: 88.965Å c: 75.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.286
Expression system: Escherichia coli BL21

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