› 2i10

X-ray diffraction
2.05Å resolution

Putative TetR transcriptional regulator from Rhodococcus sp. RHA1

Released:
DOI: 10.2210/pdb2i10/pdb
PDB entry 2i10 coloured by chain, front view.
PDB entry 2i10 coloured by chain, side view.
PDB entry 2i10 coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Osipiuk J, Evdokimova E, Kudritska M, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.26 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0RX74 (Residues: 1-198; Coverage: 100%)
Gene name: RHA1_ro09068
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NPO 1 x NPO
Ligand PGE 1 x PGE
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 44.709Å b: 53.554Å c: 178.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.202 0.245
Expression system: Escherichia coli BL21(DE3)

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