› 2i13

X-ray diffraction
1.96Å resolution

Aart, a six finger zinc finger designed to recognize ANN triplets

Released:
DOI: 10.2210/pdb2i13/pdb
PDB entry 2i13 coloured by chain, front view.
PDB entry 2i13 coloured by chain, side view.
PDB entry 2i13 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA.
Segal DJ, Crotty JW, Bhakta MS, Barbas CF, Horton NC
J Mol Biol 363 405-21 (2006)
PMID: 16963084

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115379 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Zinc finger and SCAN domain-containing protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07230 (Residues: 218-388; Coverage: 28%)
Gene names: Zfp-29, Zfp29, Zscan2
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*CP*AP*GP*AP*TP*GP*TP*AP*GP*GP*GP*AP*AP*AP*AP*GP*CP*CP*CP*GP*GP*G)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 22 nucleotides
Theoretical weight: 6.88 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*GP*CP*CP*CP*GP*GP*GP*CP*TP*TP*TP*TP*CP*CP*CP*TP*AP*CP*AP*TP*CP*T)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 22 nucleotides
Theoretical weight: 6.63 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand ZN 11 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P1
Unit cell:
a: 41.586Å b: 72.054Å c: 74.212Å
α: 75.53° β: 83.8° γ: 72.67°
R-values:
R R work R free
0.203 0.203 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Genome editing with engineered zinc finger nucleases.
Urnov et al. (2010)
5 more

31 mentions without citation

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.
Gaj et al. (2013)
30 more