› 2i14

X-ray diffraction
2.9Å resolution

Crystal structure of nicotinate-nucleotide pyrophosphorylase from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb2i14/pdb
PDB entry 2i14 coloured by chain, front view.
PDB entry 2i14 coloured by chain, side view.
PDB entry 2i14 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Entry authors: Shin DH, Kim R, Kim S-H, Berkeley Structural Genomics Center (BSGC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nicotinate phosphoribosyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 395 amino acids
Theoretical weight: 43.77 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZS9 (Residues: 1-389; Coverage: 100%)
Gene name: PF1904
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 12 x ZN
Ligand PCP 6 x PCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P31
Unit cell:
a: 111.481Å b: 111.481Å c: 178.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.247 0.282
Expression system: Escherichia coli

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