2i1s

X-ray diffraction
2.3Å resolution

Crystal Structure of Protein of Unknown Function MM3350 from Methanosarcina mazei Go1

Released:
Source organism: Methanosarcina mazei Go1
Entry authors: Nocek B, Borovilos M, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TnpR protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.96 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PRU3 (Residues: 1-188; Coverage: 100%)
Gene name: MM_3350
Sequence domains: Plasmid pRiA4b ORF-3-like protein
Structure domains: MM3350-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 62.421Å b: 90.577Å c: 107.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.243
Expression system: Escherichia coli BL21(DE3)