› 2i24

X-ray diffraction
1.35Å resolution

Crystal structure analysis of the nurse shark New Antigen Receptor PBLA8 variable domain

Released:
DOI: 10.2210/pdb2i24/pdb
PDB entry 2i24 coloured by chain, front view.
PDB entry 2i24 coloured by chain, side view.
PDB entry 2i24 coloured by chain, top view.
Source organism: Ginglymostoma cirratum
Primary publication:
Maturation of shark single-domain (IgNAR) antibodies: evidence for induced-fit binding.
Stanfield RL, Dooley H, Verdino P, Flajnik MF, Wilson IA
J Mol Biol 367 358-72 (2007)
PMID: 17258766

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ig-like domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 121 amino acids
Theoretical weight: 13.44 KDa
Source organism: Ginglymostoma cirratum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8AXH5 (Residues: 1-89, 90-112; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 53.958Å b: 53.958Å c: 79.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.214
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
22 more

14 mentions without citation

Protein-protein docking benchmark version 3.0.
Hwang et al. (2008)
13 more