› 2i2d

X-ray diffraction
2.22Å resolution

Crystal structure of LmNADK1

Released:
DOI: 10.2210/pdb2i2d/pdb
PDB entry 2i2d coloured by chain, front view.
PDB entry 2i2d coloured by chain, side view.
PDB entry 2i2d coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
NAD kinases use substrate-assisted catalysis for specific recognition of NAD.
Poncet-Montange G, Assairi L, Arold S, Pochet S, Labesse G
J Biol Chem 282 33925-34 (2007)
PMID: 17686780

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 31.05 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Y8D7 (Residues: 1-264; Coverage: 100%)
Gene names: lmo0968, nadK1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand A2D 1 x A2D
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 62.481Å b: 75.851Å c: 118.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.252
Expression system: Escherichia coli

Quick links

Citations

6 review citations

NADPH-generating systems in bacteria and archaea.
Spaans et al. (2015)
5 more

1 mention without citation

'Hot' macromolecular crystals.
Koclega et al. (2009)