› 2i2o

X-ray diffraction
1.92Å resolution

Crystal Structure of an eIF4G-like Protein from Danio rerio

Released:
DOI: 10.2210/pdb2i2o/pdb
PDB entry 2i2o coloured by chain, front view.
PDB entry 2i2o coloured by chain, side view.
PDB entry 2i2o coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Crystal structure of an eIF4G-like protein from Danio rerio.
Bae E, Bitto E, Bingman CA, McCoy JG, Wesenberg GE, Phillips GN
Proteins 78 1803-6 (2010)
PMID: 20229607

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MIF4G domain-containing protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 26.15 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EAQ1 (Residues: 2-222; Coverage: 100%)
Gene names: mif4gdb, zgc:110826
Sequence domains: MIF4G domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 317.796Å b: 40.948Å c: 40.917Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.192 0.19 0.235
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Aceti et al. (2015)

4 mentions without citation

Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins.
von Moeller et al. (2013)
3 more