› 2i2r

X-ray diffraction
3.35Å resolution

Crystal structure of the KChIP1/Kv4.3 T1 complex

Released:
DOI: 10.2210/pdb2i2r/pdb
PDB entry 2i2r coloured by chain, front view.
PDB entry 2i2r coloured by chain, side view.
PDB entry 2i2r coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
Pioletti M, Findeisen F, Hura GL, Minor DL
Nat Struct Mol Biol 13 987-95 (2006)
PMID: 17057713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer (preferred)
hetero hexadecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-178977 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Potassium voltage-gated channel subfamily D member 3 Chains: A, B, C, D, I, J, K, L
Molecule details ›
Chains: A, B, C, D, I, J, K, L
Length: 144 amino acids
Theoretical weight: 16.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62897 (Residues: 2-143; Coverage: 22%)
Gene name: Kcnd3
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Kv channel-interacting protein 1 Chains: E, F, G, H, M, N, O, P
Molecule details ›
Chains: E, F, G, H, M, N, O, P
Length: 180 amino acids
Theoretical weight: 21.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZI2 (Residues: 48-227; Coverage: 79%)
Gene names: KCHIP1, KCNIP1, VABP
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand CA 16 x CA
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 93.151Å b: 98.105Å c: 97.783Å
α: 91° β: 112.56° γ: 111.77°
R-values:
R R work R free
0.238 0.237 0.268
Expression system: Escherichia coli

Quick links

Citations

25 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
24 more

14 mentions without citation

Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne et al. (2019)
13 more