› 2i39

X-ray diffraction
2.2Å resolution

Crystal structure of Vaccinia virus N1L protein

Released:
DOI: 10.2210/pdb2i39/pdb
PDB entry 2i39 coloured by chain, front view.
PDB entry 2i39 coloured by chain, side view.
PDB entry 2i39 coloured by chain, top view.
Source organism: Vaccinia virus
Primary publication:
Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family protein.
Aoyagi M, Zhai D, Jin C, Aleshin AE, Stec B, Reed JC, Liddington RC
Protein Sci 16 118-24 (2007)
PMID: 17123957

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N1L Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 137 amino acids
Theoretical weight: 16.15 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q49PX0 (Residues: 1-117; Coverage: 100%)
Gene names: 44/47.1_rMVA_020, List025, N1L, VACAC2_037, VACCL3_037, VACV-DUKE-037, VACV_026, VACV_BRZ_SERRO2_026, VACV_CTGV_CM01_026, VACV_IOC_B141_048, VACV_IOC_B388_048, VACV_TT10_033, VACV_TT11_033, VACV_TT12_033, VACV_TT8_033, VACV_TT9_033, VAC_DPP10_037, VAC_DPP11_037, VAC_DPP12_037, VAC_DPP13_037, VAC_DPP15_037, VAC_DPP16_037, VAC_DPP17_037, VAC_DPP19_037, VAC_DPP20_037, VAC_DPP21_037, VAC_DPP25_037, VAC_DPP9_037, VAC_IHDW1_030, VAC_TKT3_017, VAC_TKT4_017, VAC_TP3_032, VAC_TP5_032, VACli_037, m8028L, mO028L, synVACV_037
Sequence domains: Poxvirus Bcl-2-like proteins
Structure domains: dsDNA poxvirus

Ligands and Environments

1 bound ligand:
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 68.64Å b: 109.99Å c: 69.58Å
α: 90° β: 110.92° γ: 90°
R-values:
R R work R free
0.202 0.202 0.247
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The BCL-2 protein family: opposing activities that mediate cell death.
Youle et al. (2008)
25 more

12 mentions without citation

How vaccinia virus has evolved to subvert the host immune response.
Bahar et al. (2011)
11 more