› 2i3p

X-ray diffraction
2.3Å resolution

K28R mutant of Homing Endonuclease I-CreI

Released:
DOI: 10.2210/pdb2i3p/pdb
PDB entry 2i3p coloured by chain, front view.
PDB entry 2i3p coloured by chain, side view.
PDB entry 2i3p coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Homing endonuclease I-CreI derivatives with novel DNA target specificities.
Rosen LE, Morrison HA, Masri S, Brown MJ, Springstubb B, Sussman D, Stoddard BL, Seligman LM
Nucleic Acids Res 34 4791-800 (2006)
PMID: 16971456

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115381 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.68 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 1-153; Coverage: 94%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*AP*AP*TP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*AP*TP*TP*TP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(*CP*GP*AP*AP*AP*TP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*AP*TP*TP*TP*GP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 43.076Å b: 68.211Å c: 87.409Å
α: 90° β: 91.42° γ: 90°
R-values:
R R work R free
0.234 0.234 0.292
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

The CRISPR tool kit for genome editing and beyond.
Adli M. (2018)
27 more

1 mention without citation

The Automatic Solution of Macromolecular Crystal Structures via Molecular Replacement Techniques: REMO22 and Its Pipeline.
Carrozzini et al. (2023)