› 2i42

X-ray diffraction
2.2Å resolution

Crystal structure of Yersinia protein tyrosine phosphatase complexed with vanadate, a transition state analogue

Released:
DOI: 10.2210/pdb2i42/pdb
PDB entry 2i42 coloured by chain, front view.
PDB entry 2i42 coloured by chain, side view.
PDB entry 2i42 coloured by chain, top view.
Source organism: Yersinia enterocolitica
Entry authors: Vijayalakshmi J, Saper MA

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.55 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15273 (Residues: 164-468; Coverage: 65%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand VO4 1 x VO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 56.39Å b: 49.66Å c: 100.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Escherichia coli BL21(DE3)

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