› 2i50

Solution NMR

Solution Structure of Ubp-M Znf-UBP domain

Released:
DOI: 10.2210/pdb2i50/pdb
PDB entry 2i50 coloured by chain, ensemble of 20 models, front view.
PDB entry 2i50 coloured by chain, ensemble of 20 models, side view.
PDB entry 2i50 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin.
Pai MT, Tzeng SR, Kovacs JJ, Keaton MA, Li SS, Yao TP, Zhou P
J Mol Biol 370 290-302 (2007)
PMID: 17512543

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 16 Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5T5 (Residues: 22-143; Coverage: 15%)
Gene names: MSTP039, USP16
Sequence domains: Zn-finger in ubiquitin-hydrolases and other protein
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
10 more

5 mentions without citation

Regulation of proteolysis by human deubiquitinating enzymes.
Eletr et al. (2014)
4 more