› 2i57

X-ray diffraction
1.97Å resolution

Crystal Structure of L-Rhamnose Isomerase from Pseudomonas stutzeri in Complex with D-Allose

Released:
DOI: 10.2210/pdb2i57/pdb
PDB entry 2i57 coloured by chain, front view.
PDB entry 2i57 coloured by chain, side view.
PDB entry 2i57 coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
The structures of L-rhamnose isomerase from Pseudomonas stutzeri in complexes with L-rhamnose and D-allose provide insights into broad substrate specificity.
Yoshida H, Yamada M, Ohyama Y, Takada G, Izumori K, Kamitori S
J Mol Biol 365 1505-16 (2007)
PMID: 17141803

Function and Biology Details

Reaction catalysed: 
L-rhamnopyranose = L-rhamnulose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181127 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase-like TIM barrel domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 438 amino acids
Theoretical weight: 48 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WH8 (Residues: 1-430; Coverage: 100%)
Gene name: L-RhI
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
Ligand AOS 4 x AOS
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 74.647Å b: 104.734Å c: 114.525Å
α: 90° β: 108° γ: 90°
R-values:
R R work R free
0.172 0.172 0.204
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Isomerases for biotransformation of D-hexoses.
Mu et al. (2015)
1 more