› 2i5e

X-ray diffraction
2.1Å resolution

Crystal Structure of a Protein of Unknown Function MM2497 from Methanosarcina mazei Go1, Probable Nucleotidyltransferase

Released:
DOI: 10.2210/pdb2i5e/pdb
PDB entry 2i5e coloured by chain, front view.
PDB entry 2i5e coloured by chain, side view.
PDB entry 2i5e coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Entry authors: Tan K, Du J, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-phospho-L-lactate guanylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.59 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8PU52 (Residues: 1-208; Coverage: 100%)
Gene names: MM_2497, cofC
Sequence domains: Guanylyl transferase CofC like
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TRS 5 x TRS
Ligand PEG 3 x PEG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 43.797Å b: 100.482Å c: 54.877Å
α: 90° β: 106.15° γ: 90°
R-values:
R R work R free
0.195 0.192 0.249
Expression system: Escherichia coli BL21

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