Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Protease and Pol protein (preferred)
PDBe Complex ID:
PDB-CPX-136847 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Reverse transcriptase/ribonuclease H
Molecule details ›
Chain: A
Length: 552 amino acids
Theoretical weight: 63.66 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 552 amino acids
Theoretical weight: 63.66 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
- Canonical:
P03366 (Residues: 600-1151; Coverage: 38%)
Sequence domains:
- Reverse transcriptase (RNA-dependent DNA polymerase)
- Reverse transcriptase thumb domain
- Reverse transcriptase connection domain
- RNase H
Pol protein
Molecule details ›
Chain: B
Length: 429 amino acids
Theoretical weight: 50.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
Length: 429 amino acids
Theoretical weight: 50.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
- Canonical: Q8UM83 (Residues: 100-349; Coverage: 72%)
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
