› 2i61

X-ray diffraction
1.2Å resolution

Depressant anti-insect neurotoxin, LqhIT2 from Leiurus quinquestriatus hebraeus

Released:
DOI: 10.2210/pdb2i61/pdb
PDB entry 2i61 coloured by chain, front view.
PDB entry 2i61 coloured by chain, side view.
PDB entry 2i61 coloured by chain, top view.
Source organism: Leiurus quinquestriatus hebraeus
Primary publication:
X-ray structure and mutagenesis of the scorpion depressant toxin LqhIT2 reveals key determinants crucial for activity and anti-insect selectivity.
Karbat I, Turkov M, Cohen L, Kahn R, Gordon D, Gurevitz M, Frolow F
J Mol Biol 366 586-601 (2007)
PMID: 17166514

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-insect depressant toxin LqhIT2 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 6.91 KDa
Source organism: Leiurus quinquestriatus hebraeus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q26292 (Residues: 22-82; Coverage: 95%)
Sequence domains: Scorpion toxin-like domain
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

5 bound ligands:
Ligand LI 3 x LI
Ligand SO4 1 x SO4
Ligand EDO 3 x EDO
Ligand CO2 1 x CO2
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 19.66Å b: 51.423Å c: 28.917Å
α: 90° β: 107.75° γ: 90°
R-values:
R R work R free
0.097 0.096 0.12
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The insecticidal potential of venom peptides.
Smith et al. (2013)
1 more

5 mentions without citation

Evolution stings: the origin and diversification of scorpion toxin peptide scaffolds.
Sunagar et al. (2013)
4 more