› 2i62

X-ray diffraction
1.8Å resolution

Mouse Nicotinamide N-methyltransferase

Released:
DOI: 10.2210/pdb2i62/pdb
PDB entry 2i62 coloured by chain, front view.
PDB entry 2i62 coloured by chain, side view.
PDB entry 2i62 coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Min J, Wu H, Zeng H, Loppnau P, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 29.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O55239 (Residues: 1-264; Coverage: 100%)
Gene name: Nnmt
Sequence domains: NNMT/PNMT/TEMT family
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 47.281Å b: 158.519Å c: 72.227Å
α: 90° β: 92.92° γ: 90°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli

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