› 2i6u

X-ray diffraction
2.2Å resolution

Crystal Structure of Ornithine Carbamoyltransferase complexed with Carbamoyl Phosphate and L-Norvaline from Mycobacterium tuberculosis (Rv1656) at 2.2 A

Released:
DOI: 10.2210/pdb2i6u/pdb
PDB entry 2i6u coloured by chain, front view.
PDB entry 2i6u coloured by chain, side view.
PDB entry 2i6u coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzyme's catalytic mechanism.
Sankaranarayanan R, Cherney MM, Cherney LT, Garen CR, Moradian F, James MN
J Mol Biol 375 1052-63 (2008)
PMID: 18062991

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine carbamoyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 307 amino acids
Theoretical weight: 33.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIT9 (Residues: 1-307; Coverage: 100%)
Gene names: MTCY06H11.21, Rv1656, argF
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand CP 3 x CP
Ligand NVA 3 x NVA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6522
Unit cell:
a: 99.149Å b: 99.149Å c: 463Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural genomics approach to drug discovery for Mycobacterium tuberculosis.
Ioerger et al. (2009)
1 more

4 mentions without citation

The TB Structural Genomics Consortium: a decade of progress.
Chim et al. (2011)
3 more