› 2i71

X-ray diffraction
1.7Å resolution

Crystal structure of a Conserved Protein of Unknown Function from Sulfolobus solfataricus P2

Released:
DOI: 10.2210/pdb2i71/pdb
PDB entry 2i71 coloured by chain, front view.
PDB entry 2i71 coloured by chain, side view.
PDB entry 2i71 coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Entry authors: Tan K, Skarina T, Onopriyenko O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system endoribonuclease Csx1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 45.84 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97YD5 (Residues: 1-377; Coverage: 100%)
Gene names: SSO1389, csx1
Sequence domains: CRISPR-associated (Cas) DxTHG family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 78.849Å b: 84.918Å c: 108.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.227
Expression system: Escherichia coli BL21

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