› 2i8e

X-ray diffraction
1.59Å resolution

Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2

Released:
DOI: 10.2210/pdb2i8e/pdb
PDB entry 2i8e coloured by chain, front view.
PDB entry 2i8e coloured by chain, side view.
PDB entry 2i8e coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats.
Beloglazova N, Brown G, Zimmerman MD, Proudfoot M, Makarova KS, Kudritska M, Kochinyan S, Wang S, Chruszcz M, Minor W, Koonin EV, Edwards AM, Savchenko A, Yakunin AF
J Biol Chem 283 20361-71 (2008)
PMID: 18482976

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas2 1 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 12.09 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97YC2 (Residues: 1-101; Coverage: 100%)
Gene names: SSO1404, cas21
Sequence domains: CRISPR associated protein Cas2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand IOD 4 x IOD
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P62
Unit cell:
a: 64.277Å b: 64.277Å c: 39.529Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli

Quick links

Citations

39 review citations

CRISPR/Cas, the immune system of bacteria and archaea.
Horvath et al. (2010)
38 more

12 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
11 more