› 2i99

X-ray diffraction
2.6Å resolution

Crystal structure of human Mu_crystallin at 2.6 Angstrom

Released:
DOI: 10.2210/pdb2i99/pdb
PDB entry 2i99 coloured by chain, front view.
PDB entry 2i99 coloured by chain, side view.
PDB entry 2i99 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human micro-crystallin complexed with NADPH.
Cheng Z, Sun L, He J, Gong W
Protein Sci 16 329-35 (2007)
PMID: 17242435

Function and Biology Details

Reaction catalysed: 
Thiomorpholine 3-carboxylate + NAD(P)(+) = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketimine reductase mu-crystallin Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 33.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14894 (Residues: 2-313; Coverage: 99%)
Gene names: CRYM, THBP
Sequence domains: Ornithine cyclodeaminase/mu-crystallin family
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 70.98Å b: 90.86Å c: 101.183Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

mu-crystallin, a NADPH-dependent T(3)-binding protein in cytosol.
Suzuki et al. (2007)
1 more

5 mentions without citation

Mutation of L-2,3-diaminopropionic acid synthase genes blocks staphyloferrin B synthesis in Staphylococcus aureus.
Beasley et al. (2011)
4 more