› 2ian

X-ray diffraction
2.8Å resolution

Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR

Released:
DOI: 10.2210/pdb2ian/pdb
PDB entry 2ian coloured by chain, front view.
PDB entry 2ian coloured by chain, side view.
PDB entry 2ian coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor.
Deng L, Langley RJ, Brown PH, Xu G, Teng L, Wang Q, Gonzales MI, Callender GG, Nishimura MI, Topalian SL, Mariuzza RA
Nat Immunol 8 398-408 (2007)
PMID: 17334368

Function and Biology Details

Reactions catalysed: 
Glycerone phosphate = methylglyoxal + phosphate
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134726 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, F, K, P
Molecule details ›
Chains: A, F, K, P
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chains: B, G, L, Q
Molecule details ›
Chains: B, G, L, Q
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Triosephosphate isomerase Chains: C, H, M, R
Molecule details ›
Chains: C, H, M, R
Length: 15 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60174 (Residues: 23-37; Coverage: 6%)
Gene names: TPI, TPI1
T cell receptor alpha chain constant Chains: D, I, N, S
Molecule details ›
Chains: D, I, N, S
Length: 202 amino acids
Theoretical weight: 22.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, J, O, T
Molecule details ›
Chains: E, J, O, T
Length: 240 amino acids
Theoretical weight: 27.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 93.474Å b: 242.866Å c: 105.222Å
α: 90° β: 111.32° γ: 90°
R-values:
R R work R free
0.225 0.221 0.296
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
21 more

21 mentions without citation

Conformational changes and flexibility in T-cell receptor recognition of peptide-MHC complexes.
Armstrong et al. (2008)
20 more