› 2ict

X-ray diffraction
1.63Å resolution

Crystal structure of the bacterial antitoxin HigA from Escherichia coli at pH 8.5. Northeast Structural Genomics TARGET ER390.

Released:
DOI: 10.2210/pdb2ict/pdb
PDB entry 2ict coloured by chain, front view.
PDB entry 2ict coloured by chain, side view.
PDB entry 2ict coloured by chain, top view.
Source organism: Escherichia coli CFT073
Primary publication:
Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.
Arbing MA, Handelman SK, Kuzin AP, Verdon G, Wang C, Su M, Rothenbacher FP, Abashidze M, Liu M, Hurley JM, Xiao R, Acton T, Inouye M, Montelione GT, Woychik NA, Hunt JF
Structure 18 996-1010 (2010)
PMID: 20696400

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-159223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized HTH-type transcriptional regulator YddM Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.65 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli
UniProt:
  • Canonical: P67700 (Residues: 1-94; Coverage: 100%)
Gene names: c1909, yddM
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 90.535Å b: 26.364Å c: 39.485Å
α: 90° β: 91.51° γ: 90°
R-values:
R R work R free
0.198 0.198 0.201
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
20 more

9 mentions without citation

Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.
Sala et al. (2014)
8 more